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Dihydropyrimidine dehydrogenase (DPD) is an enzyme that is involved in pyrimidine degradation. It is the initial and rate-limiting step in pyrimidine catabolism. It .

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DPD Deficiency simply put: the lack of a critical enzyme needed to metabolize the drug 5-FU. Under normal conditions, 80+ percent of a dose of 5-fu is quickly metabolized.

DPD enzyme activity was determined by standardizing the formation rate of [6-14 C]-5-FUH 2 to the amount protein used in the reaction mixture (i.e. nmol/min/mg protein).

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